Amylase activity of starch degrading bacteria isolated from soil

Soil contains huge diversity of microorganism which produces different types of enzymes. Amylase is one of them that hydrolyses starch into its monomer compounds, the smallest being glucose. Hence, amylase is a very prevalent enzyme produced biologically by various kinds of microorganisms and used in industrial sectors for various purposes. Soil bacteria can be isolated and commercially grown in large numbers to produce a vast amount of amylase. In addition, amylases that are extracted require optimum conditions to show greatest activity. In the present study bacteria were isolated from the garden soil and screened for amylase production on starch agar medium. Total 12 isolates were obtained by the primary screening technique from which 05 isolates were showing amylase activity. Zone clearance was determined by Gram’s iodine method. Among 5 isolates isolate 1 was showing highest amylase activity 4.70 U/ml which was considered for further identification. Isolate 1 was tentatively characterized on the basis of their cultural, morphological and biochemical characteristics, which was identified to be Bacillus sp. Further partial purification of the amylase enzyme was carried out by ammonium sulfate precipitation followed by dialysis. Optimization of different parameters was carried out for the amylase production.
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Amylase activity of starch degrading bacteria isolated from soil