Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance
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The crystal structure of theSchizosaccharomyces pombeHolliday junction resolvase Ydc2 revealed significant struc-tural homology with the Escherichia coliresolvase RuvCbut Ydc2 contains a small triple helical bundle that hasno equivalent in RuvC.Two of thea-helices that form thisbundle show homology to a putative DNA-binding motifknown as SAP.To investigate the biochemical function ofthe triple-helix domain, truncated Ydc2 mutants wereexpressed inE.coliand in fission yeast.
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Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance
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Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance
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