Improving culture conditions for poly(3- hydroxybutyrate-co-3-hydroxyvalerate) production by Bacillus sp. ND153, a bacterium isolated from a mangrove forest in Vietnam

In the present study "Improving culture conditions for poly(3- hydroxybutyrate-co-3-hydroxyvalerate) production by Bacillus sp. ND153, a bacterium isolated from a mangrove forest in Vietnam", we examined the variations in the compositions of the culture medium to optimize the conditions for PHBV production by Bacillus sp. ND153, a halotolerant bacterium strain isolated from a mangrove forest in Vietnam (Van-Thuoc et al. 2012).
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Improving culture conditions for poly(3- hydroxybutyrate-co-3-hydroxyvalerate) production by Bacillus sp. ND153, a bacterium isolated from a mangrove forest in VietnamImproving culture conditions for poly(3-hydroxybutyrate-co-3-hydroxyvalerate)production by Bacillus sp. ND153, abacterium isolated from a mangrove forestin VietnamDoan Van Thuoc & Jorge QuillaguamánAnnals of MicrobiologyISSN 1590-4261Volume 64Number 3Ann Microbiol (2014) 64:991-997DOI 10.1007/s13213-013-0736-4 1 23Your article is protected by copyright andall rights are held exclusively by Springer-Verlag Berlin Heidelberg and the Universityof Milan. This e-offprint is for personaluse only and shall not be self-archived inelectronic repositories. If you wish to self-archive your article, please use the acceptedmanuscript version for posting on your ownwebsite. You may further deposit the acceptedmanuscript version in any repository,provided it is only made publicly available 12months after official publication or later andprovided acknowledgement is given to theoriginal source of publication and a link isinserted to the published article on Springerswebsite. The link must be accompanied bythe following text: The final publication isavailable at link.springer.com”.1 23 Authors personal copyAnn Microbiol (2014) 64:991–997DOI 10.1007/s13213-013-0736-4 ORIGINAL ARTICLEImproving culture conditions for poly(3-hydroxybutyrate-co -3-hydroxyvalerate) production by Bacillus sp. ND153,a bacterium isolated from a mangrove forest in VietnamDoan Van Thuoc & Jorge QuillaguamánReceived: 30 November 2012 / Accepted: 18 October 2013 / Published online: 8 November 2013# Springer-Verlag Berlin Heidelberg and the University of Milan 2013Abstract The production of polyhydroxyalkanoate (PHA) by IntroductionBacillus sp. ND153, a bacterium strain isolated from a man-grove forest in Vietnam, was studied. Bacillus sp. ND153 was In nature, many microorganisms can accumulategrown on HM-1 medium with different carbon sources (e.g. polyhydroxyalkanoates (PHAs) as carbon and energy storageglucose, sucrose, maltose, dextrin, and starch). Glucose was materials when grown in the presence of considerablefound to be the most suitable carbon source for PHA accu- amounts of a carbon source and a limiting concentration ofmulation, whereas starch and dextrin favored cell growth over an essential nutrient. The excess carbon is metabolized by thePHA accumulation. Optimization of the culture medium for cells to PHAs that may include 3-, 4-, 5-, and 6-hydroxyacidPHA production was investigated by applying factorial de- monomers. PHAs exist as discrete granules of 0.2–0.5 μmsign, and a maximum PHA content of 79 % (w/w) was diameter, with about 3–13 granules per cell (Sudesh et al.obtained with low concentrations of NH4Cl and MgSO4 and 2000). They exhibit a high degree of polymerization anda high concentration of KH2PO4 in the medium. Propionate molecular masses of up to several million Daltonswas used as the precursor for the production of copolymer (Anderson and Dawes 1990). Different constituents have beenpoly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), and observed in PHAs isolated from different microorganismsthe amount of 3-hydroxyvalerate (3HV) in the polymer (Sudesh et al. 2000; Valappil et al. 2007). PHAs can beshowed an increasing linear trend with the increase in propi- composed of a single monomer or form a copolymer of twoonate concentration from 0.2 g l−1 to 1.0 g l−1. Thus, the or more different monomers. Poly(3-hydroxybutyrate) (PHB)production of PHBV by Bacillus sp. ND153, with 3HV was the first PHA identified and isolated from a Bacillus sp.fraction ranging from 1 mol% to 30 mol%, was noted to be (Lemoigne 1926). Since then, more than 100 distinct mono-high, and the characteristics of fast cell growth and accumu- mers have been recognized in the chemical structure of PHAslation of PHA exhibited by Bacillus sp. ND153 make it a (Philip et al. 2007). The composition and molecular weight ofpromising choice for biopolyester production. the synthesized polymer are governed by two factors: the microbial strains and the substrate provided (mainly the car- bon source) (Philip et al. 2007). PHB is the most common typeKeywords Bacillus sp. ND153 . Polyhydroxyalkanoates . of PHA synthesized by microorganisms, and is rigid andBio ...