Interrogation of site-specific CpG methylation in circulating tumor DNAs (ctDNAs) has been employed in a number of studies for early detection of breast cancer (BrCa). In many of these studies, the markers were identified based on known biology of BrCa progression, and interrogated using methyl-specific PCR (MSP), a technique involving bisulfite conversion, PCR, and qPCR.
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Prediction of blood-based biomarkers and subsequent design of bisulfite PCR-LDRqPCR assay for breast cancer detection
Prediction of blood-based biomarkers and subsequent design of bisulfite PCR-LDRqPCR assay for breast cancer detection
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