The prognostic and clinical significance of IFI44L aberrant downregulation in patients with oral squamous cell carcinoma

It is a basic task in high-throughput gene expression profiling studies to identify differentially expressed genes (DEGs) between two phenotypes. RankComp, an algorithm, could analyze the highly stable withinsample relative expression orderings (REOs) of gene pairs in a particular type of human normal tissue that are widely reversed in the cancer condition, thereby detecting DEGs for individual disease samples measured by a particular platform.
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The prognostic and clinical significance of IFI44L aberrant downregulation in patients with oral squamous cell carcinomaOuand Wu BMC Cancer (2021) 21:1327https://doi.org/10.1186/s12885-021-09058-y RESEARCH Open AccessThe prognostic andclinical significanceofIFI44L aberrant downregulation inpatientswithoral squamous cell carcinomaDemingOu1*and YingWu2  Abstract  Background:  It is a basic task in high-throughput gene expression profiling studies to identify differentially expressed genes (DEGs) between two phenotypes. RankComp, an algorithm, could analyze the highly stable within- sample relative expression orderings (REOs) of gene pairs in a particular type of human normal tissue that are widely reversed in the cancer condition, thereby detecting DEGs for individual disease samples measured by a particular platform. Methods:  In the present study, Gene Expression Omnibus (GEO) Series (GSE) GSE75540, GSE138206 were down- loaded from GEO, by analyzing DEGs in oral squamous cell carcinoma based on online datasets using the RankComp algorithm, using the Kaplan-Meier survival analysis and Cox regression analysis to survival analysis, Gene Set Enrich- ment Analysis (GSEA) to explore the potential molecular mechanisms underlying. Results:  We identified 6 reverse gene pairs with stable REOs. All the 12 genes in these 6 reverse gene pairs have been reported to be associated with cancers. Notably, lower Interferon Induced Protein 44 Like (IFI44L) expression was asso- ciated with poorer overall survival (OS) and Disease-free survival (DFS) in oral squamous cell carcinoma patients, and IFI44L expression showed satisfactory predictive efficiency by receiver operating characteristic (ROC) curve. Moreover, low IFI44L expression was identified as risk factors for oral squamous cell carcinoma patients’ OS. IFI44L downregula- tion would lead to the activation of the FRS-mediated FGFR1, FGFR3, and downstream signaling pathways, and might play a role in the PI3K-FGFR cascades. Conclusions:  Collectively, we identified 6 reverse gene pairs with stable REOs in oral squamous cell carcinoma, which might serve as gene signatures playing a role in the diagnosis in oral squamous cell carcinoma. Moreover, high expression of IFI44L, one of the DEGs in the 6 reverse gene pairs, might be associated with favorable prognosis in oral squamous cell carcinoma patients and serve as a tumor suppressor by acting on the FRS-mediated FGFR signaling. Keywords:  Oral squamous cell carcinoma, Differentially expressed genes (DEGs), RankComp, Relative expression orderings (REOs), Interferon induced protein 44 like (IFI44L) Introduction Head and neck cancer is the sixth most common malig- nant tumor in the world [1], and oral squamous cell carcinoma (OSCC) is the most common head and*Correspondence: oudeming1982@126.com neck cancer [2]. There are more than 300,000 new1 Department ofStomatology, Panyu Central Hospital, cases of OSCC worldwide every year, and more thanGuangzhou511400, China 140,000 patients die from OSCC every year [2, 3]. MoreFull list of author information is available at the end of the article © The Author(s) 2021. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission ...