Amperometric urea biosensor based on immobilized urease on polypyrrole and macroporous polypyrrole modified Pt electrode

Biosensing of urea by a biosensor as a direct detection method at ambient temperature and pressure instead of chromatography leads to a significant reduction in processing costs. Amperometric biosensors based on urease immobilization on macroporous polypyrrole (MPPy) and pyrrole on the surface of a Pt electrode were developed. Applying cyclic voltammetry (CV), we demonstrated the synthesis of MPPy using monodispersed polystyrene spheres (460 nm) as a template. CV and chronoamperometric studies were conducted to evaluate the electrochemical current of the modified electrodes.
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Amperometric urea biosensor based on immobilized urease on polypyrrole and macroporous polypyrrole modified Pt electrode